Fig. 2.NEAT1 siRNAs induce apoptosis in A549 and Calu3 cells. (A) Effects of NEAT1 siRNAs on A549 cell apoptosis evaluated by fluorescence microscopy after Hoechst 33342/PI staining. (B) Effects of NEAT1 siRNAs on Calu3 cell apoptosis evaluated by fluorescence microscopy after Hoechst 33342/ PI staining. (C) Effects of NEAT1 siRNAs on A549 cell apoptosis per caspase 3/7 activity evaluated by the Apo-ONE® Homogeneous Caspase-3/7 Assay. (D) Effects of NEAT1 siRNAs on Calu3 cell apoptosis per caspase 3/7 activity evaluated by the Apo-ONE® Homogeneous Caspase-3/7 Assay. *P<0.05; **P<0.01; ***P<0.001. (E) NEAT1 siRNAs inhibit the viability and stimulated apoptosis of A549 and Calu3 cells. Hoechst 33342-positive/PI-negative (white arrow): viable cells; Hoechst 33342-positive/PI-negative with blue fragmentation in the cells (red arrow): early apoptotic cells; Hoechst 33342-positive/PI-positive with red fragmentation in the cells (pink arrow): late apoptotic cells; PI-positive with debris signals (yellow arrow): necrotic cells.